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Catalog Number | orb420415 |
---|---|
Category | Antibodies |
Description | Histone H3 K36me3 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | DOT, IF, IHC, Multiplex Assay, WB |
Reactivity | C. elegans, Human, Mouse |
Isotype | IgG |
Immunogen | Histone H3 [Trimethyl Lys36] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 36 of human Histone H3.2. |
Concentration | 1.0 mg/mL |
Dilution range | IHC: 1:500, IF: 1:500, WB: 1:500 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Histone H3 [Trimethyl Lys36] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q71DI3 |
NCBI | NP_001005464 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Histone H3 trimethyl Lys36 antibody, H Read more... |
Note | For research use only |
Application notes | Anti-Histone H3 [Trimethyl Lys36] antibody is tested for Western Blot, Dot Blot, and Immunofluorescence. This antibody is useful in Chromatin Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals. |
Expiration Date | 12 months from date of receipt. |
Dot Blot of Rabbit Histone H3 [Trimethyl Lys36] Antibody. Lane 1: K36. Lane 2: K36Me1. Lane 3: K36Me2. Lane 4: K36Me3. Lane 5: K36Ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Trimethyl Lys36] antibody at 0.5 µg/ml for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
Immunofluorescence of Anti-Histone H3 K36 me3: Histone H3 Me3 Lys36 antibody was tested at 1:500 in HeLa cells with FITC (green). Cells and nuclei were counterstained with DAPI (blue) and DyLight 550 (red). (40X).
Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys36] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys36] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys36] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys36] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
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