Histone H3 K27me3 antibody

• Catalog Number: orb420432
• Category: Antibodies
• Description: Histone H3 K27me3 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Histone H3 [Trimethyl Lys27] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27me3 site of human Histone H3.
• Concentration: 0.93 mg/ml
• Dilution range: ELISA: 1: 10,000, IF: 1:100, IP: 1:100, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Histone H3 [Trimethyl Lys27] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [Lys27 me3] from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q71DI3
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Histone H3 trimethyl Lys27 antibody, H
• Note: For research use only
• Application notes: Anti-Histone H3 [Trimethyl Lys27] antibody is tested for Dot Blot, IF, and Western Blot. This antibody is useful for ELISA and immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4kDa corresponding to the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Histone H3 K27 Me3 Antibody. Lane 1: Non Modified Peptide. Lane 2: Histone H3 K27-Me1 Peptide. Lane 3: Histone H3 K27-Me2 Peptide. Lane 4: Histone H3 K27-Me3 Peptide. Lane 5: Histone H3 K27-Ac Peptide. Dilution: 0.1 µg/mL. Blocking: 5% Blotto at RT 30 min. Exposure time: 13 sec.

Immunofluorescence of Histone H3 K27me3 Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 K27me3 antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10000 for 45 min at RT. Localization: Histone H3 K27me3 is nuclear and chromosomal. Staining: Histone H3 K27me3 is expressed green and the nuclei are counterstained blue with DAPI.

Western Blot of Histone H3 K27me3 Antibody. Western Blot analysis against untreated cell extracts. Lane 1: Molecular Weight Marker. Lane 2: HeLa cell lysates (p/n orb348668). Lane 3: 3T3 cell lysates (p/n orb348714). Lane 4: PC12 cell lysates (p/n orb348733). Lane 5: A431 cell lysate (p/n orb348665). Primary antibody: Histone H3 K27me3 antibody at 1.0 µg/mL for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10000 for 45 min at RT. Block: (p/n orb348644) BlockOut overnight at 4°C. Predicted/Observed size: 15 kDa for Histone H3 K27Me3. Other band(s): none.