Histone H3 (Di Methyl K36) Rabbit Polyclonal Antibody (Cy5.5)

• Catalog Number: orb1046643
• Category: Antibodies
• Description: Histone H3 (Di Methyl K36) Rabbit Polyclonal Antibody (Cy5.5)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF
• Predicted Reactivity: Bovine, Drosophila, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of Di Methyl K36 GV(Di Methyl-K)KP
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1μg /Test, IF=1:100-500
• Conjugation: Cy5.5
• Target: H3C1
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Histone H3(Di Methyl Lys36); H3K36me2; Di methyl H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: A431. Primary Antibody (green line): Rabbit Anti-Histone H3 (Di Methyl K36) antibody (orb1046643), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Histone H3 (Di Methyl K36) antibody (orb1046643), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: NIH/3T3. Primary Antibody (green line): Rabbit Anti-Histone H3 (Di Methyl K36) antibody (orb1046643), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Tissue/Cell: A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal rabbit serum) at 37°C for 20 min, Incubation: Anti-Histone H3 (Di Methyl K36) Polyclonal Antibody, conjugated 1:50, 2 hours at 37°C, DAPI (blue) was used to stain the cell nuclei.