Histone H2B (AcK34) antibody

• Catalog Number: orb1289927
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to Histone H2B
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K34 of human Histone H2B protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI; HIST1H2BD; HIST1H2BH; HIST1H2BK; HIST1H2BL; HIST1H2BM; HIST1H2BN; HIST2H2BF; H2BFS
• Entrez: 8344, 8341, 319182, 440689, 319179, 85236, 8342, 8340, 8339, 8345, 8343, 8347, 319184, 8346, 3017
• UniProt ID: Q99880, Q99877, Q99879, Q5QNW6, Q93079, O60814, P62807, Q6ZWY9, P58876, P57053, Q8CGP1, Q64478
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: HIST1H2BC; H2BFL; HIST1H2BE; H2BFH; HIST1H2BF; H2B
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H2B (Acetyl-K34) expression in A549 (A), BV2 (B), PC12 (C) whole cell lysates. (Predicted band size: 13 kD; Observed band size: 15 kD)

Direct ELISA antibody dose-response curve using Anti-Histone H2B (Acetyl-K34) Antibody. Antigen (Acetyl-peptide and non-acetyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.