Histone H2B (Acetyl K11) Mouse Monoclonal Antibody

• Catalog Number: orb1152019
• Category: Antibodies
• Description: Histone H2B (Acetyl K11) Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: H2C1
• Tested applications: WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Histone H2B (Acetyl K11)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 14 kDa
• Target: H2BC3
• UniProt ID: P33778
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: H2BK11ac; Histone H2B (Acetyl Lys11); Acetyl Histo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + Sodium butyrate (30mM, 4hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14 kDa, Observed MW: 14 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:500, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: Mouse liver, Mouse spleen, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14 kDa, Observed MW: 14 kDa.

IP of HeLa + Sodium butyrate (30mM, 4hr) cells extracts, IP ab incubation condition: orb1152019, 4°C overnight, 1:25, 1:100 dilution, WB primary ab incubation condition: orb1152019, room temperature 2h, 1:2000 dilution, Secondary ab: Anti-Mouse IgG for IP (HRP), Blocking buffer and Concentration: 5% NFDM/TBST, Diluting buffer and Concentration: 5% NFDM/TBST, Lane 1: 5% Input, Lane 2: IP with orb1152019 (1:25), Lane 3: IP with orb1152019 (1:100), Observed MW: 14 kDa, Exposure time: 60 s.