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Catalog Number | orb768586 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Histone H1 (phospho-Thr17) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Monkey, Mouse |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H1 around the phosphorylation site of Thr17. AA range:1-50 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 31 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-HIST1H1B antibody, anti-H1F5 antibody, anti-H Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of Hela cell. 1, Histone H1 (phospho Thr17) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). NSE Monoclonal Antibody (13E2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
Western blot analysis of SKOV3 using p-Histone H1.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Histone H1 (Phospho-Thr17) Antibody.
Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using Histone H1 (Phospho-Thr17) Antibody. The picture on the right is blocked with the phospho peptide.
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Monkey, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
Rabbit | |
Recombinant | |
Biotin |
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