Histone H1 Antibody

• Catalog Number: orb606517
• Category: Antibodies
• Description: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: r1415-1
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2a, kappa
• Immunogen: Nuclei of human leukemia biopsy cells were used as the immunogen for the recombinant Histone H1 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Histone H1 antibody is available for research use only.
• UniProt ID: P07305
• Storage: Store the recombinant Histone H1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.; 2. View the non-recombinant version of this < a href=../tds/histone-antibody-1415-1-v2567>Histone H1 antibody.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil with recombinant Histone H1 antibody (clone r1415-1). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat pancreas with recombinant Histone H1 antibody (clone r1415-1). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Histone H1 antibody (clone r1415-1) as confirmation of integrity and purity.

Flow cytometry testing of permeabilzed human HeLa cells with recombinant Histone H1 antibody (clone r1415-1); Red=isotype control, Blue= recombinant Histone H1 antibody.

Immunofluorescent staining of PFA-fixed human HeLa cells with recombinant Histone H1 antibody (clone r1415-1, green) and Phalloidin (red).

Western blot testing of human heart lysate with recombinant Histone H1 (clone R1415-1). Observed molecular weight ~22/30-33 kDa (unmodified/modified).