Histone H1 Antibody

• Catalog Number: orb386252
• Category: Antibodies
• Description: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: HH1/1784R
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the rabbit monoclonal recombinant Histone H1 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Histone H1 antibody is available for research use only.
• UniProt ID: P07305
• Storage: Store the recombinant Histone H1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pancreas and recombinant Histone H1 antibody (clone HH1/1784R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

Immunofluorescent staining of permeabilized human HeLa cells with recombinant Histone H1 antibody (green, clone HH1/1784R) and Phalloidin.

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Histone H1 antibody (clone HH1/1784R); Red=isotype control, Blue= recombinant Histone H1 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant Histone H1 antibody (clone HH1/1784R) as confirmation of integrity and purity.

Western blot testing of human heart lysate with recombinant Histone H1 (clone HH1/1784R). Observed molecular weight ~22/30-33 kDa (unmodified/modified).