HIST1H4A Antibody (C-term)

• Catalog Number: orb1788362
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: C. elegans, Drosophila, Other, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: 71-103 aa
• Dilution range: WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 11367 Da
• Target: This HIST1H4A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 71-103 amino acids from the C-terminal region of human HIST1H4A.
• UniProt ID: P62805
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Histone H4, HIST1H4A, H4/A, H4FA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded M. skin section using HIST1H4A Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded R. skin section using HIST1H4A Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. skin section using HIST1H4A Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of MCF-7 cells using HIST1H4A Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded M. esophagus section using HIST1H4A Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. esophagus section using HIST1H4A Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from A431, mouse NIH/3T3, L929, rat C6, Hela cell line (from left to right), using HIST1H4A Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.