HIST1H3A (Ab-27) Antibody

• Catalog Number: orb517083
• Category: Antibodies
• Description: HIST1H3A (Ab-27) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Peptide sequence around site of Lys (27) derived from Human Histone H3.1
• Dilution range: WB:1:500-1:5000, IHC-P:1:10-1:100, IF:1:50-1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HIST1H3A
• UniProt ID: P68431
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Alternative names: H3 histone family member E pseudogene antibody; H3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation Hela (4*106) were treated with Benzanase, sonicated, and immunoprecipitated with 5ug anti-HIST1H3A or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the

Western blot aanalysis of Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysateusing HIST1H3A (Ab-27) antibody

Immunofluorescence staining of HepG2 cells using HIST1H3A (Ab-27) antibody

Immunohistochemistry of paraffin-embedded human glioma tissue using HIST1H3A (Ab-27) antibody