HIST1H2BJ Antibody

• Catalog Number: orb1270634
• Category: Antibodies
• Description: HIST1H2BJ Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Monkey, Mouse, Rat, Xenopus, Zebrafish
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This HIST1H2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the Central region of human HIST1H2BJ.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 14 kDa
• Target: HIST1H2BJ
• UniProt ID: P06899
• NCBI: P06899
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Histone H2B type 1-J, Histone H2B1, Histone H2Br,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in CEM, HL-60, NCI-H460 cell line lysates (35 ug/lane).

Western blot analysis of lysates from ZF4 cell line, Zebrafish, zebra fish muscle tissue lysate (from left to right), using HIST1H2BJ Antibody at 1:1000 at each lane.

HIST1H2B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.