His tag Antibody

• Catalog Number: orb1294276
• Category: Antibodies
• Description: Rabbit polyclonal antibody to His tag
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Isotype: Rabbit-IgG
• Immunogen: KLH-conjugated HHHHHH (6X His)
• Dilution range: Western Blot 1:2000-1:4000 Immunofluorescence 1:500-1:1000
• Conjugation: Unconjugated
• Target: His tag
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

His-tag antibody detects His-tagged protein by western blot analysis. The membrane was then blocked for an hour before being incubated with orb1294276 overnight at 4°C. His-tag antibody (orb1294276) diluted at 1:2000.