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Catalog Number | orb548525 |
---|---|
Category | Antibodies |
Description | His Tag Antibody (monoclonal, 19b10) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 19b10 |
Reactivity | All |
Isotype | Mouse IgG2b |
Immunogen | A synthetic peptide corresponding to a sequence of His Tag (HHHHHH). |
Concentration | 0 |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of His Tag using anti-His Tag antibody (orb548525). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse His Tag protein 10ng, Lane 1: recombinant mouse His Tag protein 5ng, Lane 1: recombinant mouse His Tag protein 2.5ng, Lane 1: recombinant mouse His Tag protein 1.25ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-His Tag antigen affinity purified monoclonal antibody (Catalog # orb548525) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system.
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