His Tag (2A2) Mouse mAb Antibody

• Catalog Number: orb1562825
• Category: Antibodies
• Description: His Tag (2A2) Mouse mAb Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2A2-4H2-10D8
• Tested applications: ICC, IP, WB
• Reactivity: Other
• Isotype: IgG1
• Immunogen: Synthetic peptide: HHHHHH conjugated to KLH.
• Concentration: 1 mg/ml
• Dilution range: WB: 1/1000-1/10000 IF: 1/100-1/500 IP: 1/200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: 6 His epitope tag; Hexa His tag; HHHHHH epitope ta
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

2ug His fusion protein+ Primary antibody dilution at 1) 1:5000. 2) 1:10000.

Immunofluorescence analysis of His Tag (2A2) in 293 cells transfected with a Histag protein using His Tag (2A2) antibody (red), and DAPI (blue).

Immunoprecipitation analysis of Flag Tag (7E1) in 1: untransfected 293 cell lysates 2: transfected 293 cell lysates with Histag fusion protein 3: IP (untransfected 293+antiHis mAb+ Protein G agarose) 4: IP (transfected 293+ normal mouse IgG+Protein G agarose) 5: IP (transfected 293+antiHis mAb+ Protein G agarose).