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Catalog Number | orb2024290 |
---|---|
Category | Proteins |
Description | Hirudin Protein |
Form/Appearance | Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 and 2% mannitol. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC(b) Analysis by SDS-PAGE. |
Source | Pichia pastoris |
Biological Activity | The biological activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. |
Storage | Reconstitution: It is recommended to reconstitute the lyophilized Hirudin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.Storage: Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -1°C. Upon reconstitution Hirudin should be stored at 4C between 2-7 days and for future use below -1°C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
Buffer/Preservatives | Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 and 2% mannitol. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
Alternative names | Hirudin, Hirudin Recombinant Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 96 % by SDS-PAGE and HPLC analyses. | |
6.7 kDa |
Greater than 90% as determined by SDS-PAGE. | |
9 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
34 kDa | |
E.coli |
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