HIRA Antibody

• Catalog Number: orb1255824
• Category: Antibodies
• Description: HIRA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human HIRA (NP_003316.3).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:100 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 112kDa
• Target: HIRA
• UniProt ID: P54198
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Protein HIRA, TUP1-like enhancer of split protein
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HIRA antibody (orb1255824) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human gastric cancer using HIRA antibody (orb1255824) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using HIRA antibody (orb1255824) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using HIRA antibody (orb1255824) at dilution of 1:100 (40x lens).