HighFidelity IR680LT PCR Labeling Kit

• Catalog Number: orb1733327
• Category: Tools
• Description: HighFidelity IR680LT PCR Labeling Kit is designed to produce ran-domly IR680LT-modified DNA probes by PCR. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4 kbp is feasible. dUTP-XX-IR680LT is efficiently incorporated into DNA as substitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taqpolymerase and a proofreading enzyme. 50% dUTP-XX-IR680LT substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of dUTP-XX-IR680LT/dTTP ra-tio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions (S-Pack) or 50 labeling reactions (L-Pack) of 20 µl each (50% dUTP-XX-IR680LT substitution, 100 µM dATP/dGTP/dCTP, 50 µM dTTP, 50 µM dUTP-XX-IR680LT).
• SMILES: P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H](C[C@@H]1O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCc1ccc(cc1)/C(=C\C=C\1/C(c2c(N1C)ccc1c2cc(cc1S(=O)(=O)O)S(=O)(=O)O)(C)CCCS(=O)(=O)O)/C=C/C1=[N+](c2c(C1(C)CCCS(=O)(=O)O)c1c(cc2)c(cc(c1)S(=O)(=O)O)S(=O)(=O)[O-])C
• Storage: store at -20 °C. avoid freeze/thaw cycles, store dark
• Note: For research use only
• Application notes: Spectroscopic Propertie: λexc 679 nm, λem 694 nm, ε 190.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
• Expiration Date: 12 months from date of receipt.