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Catalog Number | orb653773 |
---|---|
Category | Tools |
Description | HighFidelity Cy5 PCR Labeling Kit is designed to produce randomly Cy5-modified DNA probes by PCR. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4 kbp is feasible. dUTP-Cy5 is efficiently incorporated into DNA as substitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taqpolymerase and a proofreading enzyme. 50% dUTP-Cy5 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of dUTP-Cy5/dTTP ratio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions (S-Pack) or 50 labeling reactions (L-Pack) of 20 µl each (50% dUTP-Cy5 substitution, 100 µM dATP/dGTP/dCTP, 50 µM dTTP, 50 µM dUTP-Cy5). |
SMILES | C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCN1/C(=C/C=C/C=C/C2=[N+](CC)c3c(C2(C)C)cc(S(=O)(=O)[O-])cc3)/C(c2c1ccc(c2)S(=O)(=O)O)(C)C |
Storage | store at -20 °C |
Note | For research use only |
Application notes | Spectroscopic Propertie: λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5). |
Expiration Date | 12 months from date of receipt. |
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