HighFidelity AF647 PCR Labeling Kit

• Catalog Number: orb1176615
• Category: Tools
• Description: HighFidelity AF647 PCR Labeling Kit is designed to produce randomly AF647-modified DNA probes by PCR. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4 kbp is feasible. dUTP-XX-AF647 is efficiently incorporated into DNA as substi-tute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taqpoly-merase and a proofreading enzyme. 50% dUTP-XX-AF647 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of dUTP-XX-AF647/dTTP ratio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions (S-Pack) or 50 labeling reactions (L-Pack) of 20 µl each (50% dUTP-XX-AF647 substitution, 100 µM dATP/dGTP/dCTP, 50 µM dTTP, 50 µM dUTP-XX-AF647).
• SMILES: P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H](C[C@@H]1O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCC[N+]1=C(C(C)(c2cc(ccc12)S(=O)(=O)O)CCCCS(=O)(=O)[O-])/C=C/C=C/C=C\1/N(c2ccc(cc2C1(C)C)S(=O)(=O)O)CCCS(=O)(=O)O
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Spectroscopic Propertie: λexc 648 nm, λem 671 nm, ε 270.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
• Expiration Date: 12 months from date of receipt.