HighFidelity AF594 PCR Labeling Kit

• Catalog Number: orb1176616
• Category: Tools
• Description: HighFidelity AF594 PCR Labeling Kit is designed to produce randomly AF594-modified DNA probes by PCR. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4 kbp is feasible. dUTP-XX-AF594 is efficiently incorporated into DNA as substi-tute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taqpoly-merase and a proofreading enzyme. 50% dUTP-XX-AF594 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of dUTP-XX-AF594/dTTP ratio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions (S-Pack) or 50 labeling reactions (L-Pack) of 20 µl each (50% dUTP-XX-AF594 substitution, 100 µM dATP/dGTP/dCTP, 50 µM dTTP, 50 µM dUTP-XX-AF594).
• SMILES: C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCNC(=O)CCCCCNC(=O)c1ccc(C2=c3cc4C(=CC(C)(C)[N+](=c4cc3Oc3cc4N(C)C(C)(C)C=C(CS(=O)(=O)O)c4cc23)C)CS(=O)(=O)O)c(c1)C(=O)[O-].C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCNC(=O)CCCCCNC(=O)c1ccc(c(C2=c3cc4C(=CC(C)(C)[N+](=c4cc3Oc3cc4N(C)C(C)(C)C=C(CS(=O)(=O)O)c4cc23)C)CS(=O)(=O)O)c1)C(=O)[O-]
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Spectroscopic Propertie: λexc 590 nm, λem 617 nm, ε 92.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
• Expiration Date: 12 months from date of receipt.