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Catalog Number | orb1326955 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to HIF-1 alpha (HIF1A) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | OTI2D5 |
Tested applications | WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG1 |
Immunogen | Human recombinant protein fragment corresponding to amino acids 531-826 of human HIF1a (NP_001521) produced in E.coli. |
Dilution range | WB 1:500 |
Conjugation | Unconjugated |
MW | 92.5 kDa |
Target | HIF1A |
UniProt ID | Q16665 |
NCBI | NM_001530 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Application notes | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIF1A ( Right lane) using HIF-1 alpha (HIF1A) antibody
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Goat, Guinea pig, Rabbit, Rat | |
Gallus, Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Bovine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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