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Catalog Number | orb500810 |
---|---|
Category | Antibodies |
Description | HIF-1 Alpha antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IHC-P, WB |
Predicted Reactivity | Bovine, Canine, Mouse, Porcine, Rabbit |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from middle of human HIF-1 Alpha (341-450/826 aa) |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1μg/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 92 kDa |
Target | HIF-1 Alpha |
UniProt ID | Q16665 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | ARNT interacting protein; ARNT-interacting protein Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry of Hela (blue).Primary Antibody (green line): Rabbit Anti- HIF-1 Alpha antibody; Dilution:1μg /10^6 cells.
Flow cytometry of Mouse spleen.Primary Antibody (green line): Rabbit Anti-HIF-1 Alpha antibody; Dilution:2μg /10^6 cells.
ICC analysis of Hela cell using HIF-1 Alpha polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.
Western blot:Lane 1:Hela (Human) Cell;Lane 2:A431 (Human) Cell;Lane 3:U251 (Human) Cell;Lane 4:HepG2 (Human) Cell;Primary:Anti-HIF-1 Alpha at 1:1000 dilution.
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Goat, Guinea pig, Rabbit, Rat | |
Gallus, Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Bovine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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