HIF-1 Alpha antibody

• Catalog Number: orb500743
• Category: Antibodies
• Description: HIF-1 Alpha antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC-P, WB
• Predicted Reactivity: Gallus, Mouse
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HIF-1 Alpha (661-760/826 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1μg/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 92 kDa
• Target: HIF-1 Alpha
• UniProt ID: Q16665
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 15mM Sodium Azide, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4
• Alternative names: ARNT interacting protein; ARNT-interacting protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ICC analysis of U-87MG cell using HIF-1 Alpha polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of Rat kidney using HIF-1 Alpha Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of Human kidney using HIF-1 Alpha Polyclonal Antibody at 1:200 dilution.

Western blot:A431(Human) Cell;U2os(Human) Cell;HepG2(Human) Cell;Primary:Anti-HIF-1 Alpha at 1:1000 dilution.