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Catalog Number | orb345696 |
---|---|
Category | Antibodies |
Description | HICE1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Anti-Hice1 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region of amino acids containing serine 70 of human Hice1. |
Concentration | 0.45 mg/mL |
Dilution range | ELISA: 1:30,000-1:45,000, WB: 0.45 µg/mL |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Hice1 Affinity purified antibody is directed against human Hice1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known. |
Conjugation | Unconjugated |
UniProt ID | Q9BT25 |
NCBI | NP_219485.1 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-HICE1 antibody, HICE-1, HICE 1, HAUS8, Read more... |
Note | For research use only |
Application notes | Hice1 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kDa in size corresponding to human Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit anti-HICE1 antibody. Lane 1: 293T Null. Lane 2: 293T WT Hice1. Lane 3: 293T S70A Hice1. Load: 14 µL per lane. Primary antibody: HICE1 antibody at 1.24 ug/ml overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 48 kDa, 48 kDa for HICE1. Other band(s): HICE1 splice variants and isoforms.
Western Blot of Rabbit Anti-Hice1 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 µg per lane. Primary antibody: Hice1 antibody at 0.5 µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1. Other band(s): none.
ICC, IHC-P, IP, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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