HHLA2 Antibody

• Catalog Number: orb1254914
• Category: Antibodies
• Description: HHLA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human HHLA2 (NP_009003.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 45-50kDa
• Target: HHLA2
• UniProt ID: Q9UM44
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: HERV-H LTR-associating protein 2, Human endogenous
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HHLA2 antibody (orb1254914) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human colon using HHLA2 antibody (orb1254914) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung using HHLA2 antibody (orb1254914) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using HHLA2 antibody (orb1254914) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using HHLA2 antibody (orb1254914) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using HHLA2 antibody (orb1254914) at dilution of 1:100 (40x lens).