HGF Rabbit Polyclonal Antibody

• Catalog Number: orb10794
• Category: Antibodies
• Description: HGF Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HGF alpha (201-300/723aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:200-500, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54/80 kDa
• Target: HGF
• UniProt ID: P14210
• RRID: AB_10930419
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HGF alpha; HGFA; F TCF; Hepatocyte growth factor;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhao, Yong et al. Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production Theriogenology, 89, 280-288 (2017)

PubMed: 28043364

Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (HGF) Polyclonal Antibody, Unconjugated (orb10794) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Lane 1: Rat Placenta tissue lysates, Lane 2: Rat Cerebellum tissue lysates, Primary: Anti-HGF (orb10794) at 1/200 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54/80 kDa, Observed band size: 60 kDa.