HFM1 Antibody

• Catalog Number: orb1255742
• Category: Antibodies
• Description: HFM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HFM1 (NP_001017975.4).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 130kDa
• Target: HFM1
• UniProt ID: A2PYH4
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Probable ATP-dependent DNA helicase HFM1, SEC63 do
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of K-562 cells, using HFM1 antibody (orb1255742) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.