HEY1 antibody

• Catalog Number: orb538221
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HEY1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 114-129 of human HEY1 (NP_001269780.1).
• Dilution range: WB: 1:500-1:2000
• Conjugation: Unconjugated
• MW: 33kDa
• UniProt ID: Q9Y5J3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Alternative names: HEY1, BHLHb31, CHF2, HERP2, HESR1, HRT-1, OAF1, hH
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A431 cells using HEY1 Rabbit pAb (orb538221) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using HEY1 Rabbit pAb (orb538221) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.