HEXIM1 antibody

• Catalog Number: orb19364
• Category: Antibodies
• Description: Goat polyclonal antibody to HEXIM1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 40.6
• Target: HEXIM1
• Entrez: 10614
• Protein Sequence: HRQQERAPLSKFGD
• RRID: AB_10753555
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti HEXIM1 antibody, anti hexamethylene bis-aceta
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 65kDa band observed in HeLa cell lysate and using recombinant HEXIM1 protein. Data kindly provided by David Price and Jeff Cooper, University of Iowa, USA. We find an approx. 40kDa band in Human Heart lysates (calculated MW of 40.6kDa according to NP_006451.1). Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19364 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb19364 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb19364 (0.1µg/ml) staining of Human (A) and Mouse (B) Heart lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Western blot analysis of HeLa cell lysate and recombinant HEXIM1 using HEXIM1 antibody

Western blot analysis of Human and Mouse Heart lysates using HEXIM1 antibody

Immunofluorescence analysis of A431 cells using HEXIM1 antibody

Immunofluorescence analysis of HeLa cells using HEXIM1 antibody