HES1 antibody

• Catalog Number: orb574166
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HES1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Predicted Reactivity: Bovine, Equine, Guinea pig, Rabbit, Rat, Zebrafish
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HES1
• Concentration: 1.0 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 30 kDa
• Target: HES1
• UniProt ID: Q14469
• Protein Sequence: Synthetic peptide located within the following region: NTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSR
• NCBI: NP_005515
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: HHL, HRY, HES-1, bHLHb39
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. Canonical 30 kDa isoform is identified, and a second isoform of 23 kDa is also present in some samples in N36.

HES1 antibody - N-terminal region (orb574166) validated by WB using Mouse embryo tissue at 1:800.

HES1 antibody - N-terminal region (orb574166) validated by WB using transfected Ls174Tcell lysate at 1 ug/ml.

K562

Rabbit Anti-HES1 Antibody, Paraffin Embedded Tissue: Human cardiac cell, Cellular Data: Epithelial cells of renal tubule, Antibody Concentration: 4.0-8.0 ug/ml, Magnification: 400X.

transfected Ls174T cell lysate at 50 ug/ml.

WB Suggested Anti-HES1 Antibody Titration: 1.25 ug/ml, Positive Control: Raji cell lysate, HES1 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells.