HER2 antibody

• Catalog Number: orb1294317
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HER2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human HER2 / ERBB2
• Dilution range: Western Blot 1:500–1:1000 Immunofluorescence 1:200–1:400 Immunohistochemistry (Paraffin) 1: 200-1:250
• Conjugation: Unconjugated
• Target: HER2 / ERBB2
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-HER2/ERBB2 antibody - at 1/500 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with orb1294317 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294317 Anti-HER2/ERBB2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling HER2/ERBB2 with orb1294317 at 1/200.

Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling HER2/ERBB2 with orb1294317 at 1/200.

Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling HER2/ERBB2 with orb1294317 at 1/200.