HER2 Antibody / ErbB2 / Extracellular region

• Catalog Number: orb699699
• Category: Antibodies
• Description: Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: ZR5
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Rabbit
• Immunogen: A recombinant protein encoding the extracellular domain of human c-erbB2 was used as the immunogen for the recombinant HER2 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant HER2 antibody is available for research use only.
• UniProt ID: P04626
• Storage: Store the recombinant HER2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant HER2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ZR5). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ZR5). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human MCF7 cells with HER2 antibody (clone ZR5); Red=isotype control, Blue= HER2 antibody.

Immunofluorescent staining of PFA-fixed human MCF7 cells with HER2 antibody (clone ZR5, green) and Phalloidin (red).