HER2 Antibody / ErbB2 / Extracellular region

• Catalog Number: orb699699
• Category: Antibodies
• Description: Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: ZR5
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Rabbit
• Immunogen: A recombinant protein encoding the extracellular domain of human c-erbB2 was used as the immunogen for the recombinant HER2 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant HER2 antibody is available for research use only.
• UniProt ID: P04626
• Storage: Store the recombinant HER2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant HER2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of PFA-fixed human MCF7 cells with HER2 antibody (clone ZR5); Red=isotype control, Blue= HER2 antibody.

Immunofluorescent staining of PFA-fixed human MCF7 cells with HER2 antibody (clone ZR5, green) and Phalloidin (red).

IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ZR5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ZR5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.