HER2 Antibody / ErbB2 / Extracellular region

• Catalog Number: orb1825179
• Category: Antibodies
• Description: Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This mAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rERBB2/6637
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant protein encoding the extracellular domain of the human protein was used as the immunogen for the recombinant ErbB2 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant ErbB2 antibody is available for research use only.
• UniProt ID: P04626
• Storage: Aliquot the recombinant ErbB2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human breast carcinoma tissue stained with recombinant ErbB2 antibody (clone rERBB2/6637) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma tissue stained with recombinant ErbB2 antibody (clone rERBB2/6637) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC testing of FFPE human brain tissue with recombinant ErbB2 antibody (clone rERBB2/6637) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.