HER2 Antibody / ErbB2 / Extracellular region

• Catalog Number: orb1151705
• Category: Antibodies
• Description: Rabbit monoclonal antibody to HER2
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: ERBB2/4376R
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Recombinant extracellular domain of human c-erbB2/HER2 was used as the immunogen for the recombinant ErbB2 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant ErbB2 antibody is available for research use only.
• UniProt ID: P04626
• Storage: Aliquot the recombinant ErbB2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant ErbB2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human breast carcinoma tissue with recombinant ErbB2 antibody (clone ERBB2/4376R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma tissue with recombinant ErbB2 antibody (clone ERBB2/4376R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human tonsil tissue with recombinant ErbB2 antibody (ERBB2/4376R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using recombinant ErbB2 antibody (clone ERBB2/4376R). These results demonstrate the foremost specificity of the ERBB2/4376R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.