Hemoglobin beta S antibody

• Catalog Number: orb420237
• Category: Antibodies
• Description: Hemoglobin beta S antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 23E5.H6.G6.C1.H7.F7.G9.F6
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Anti-Hemoglobin beta S Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb β-subunit conjugated to KLH.
• Concentration: < p>1.0mg/ml

• Dilution range: WB: 1ug/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: This protein A purified mouse monoclonal antibody reacts specifically with human HbS beta sickle isoform. Anti-HbS is purified from tissue culture supernatant by protein A purification. Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, and Hylobates lar. This antibody does not react with the HbA, HbF, HbC, or HbA-2 isoform.
• Conjugation: Unconjugated
• UniProt ID: P68871
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-HbS antibody, mouse anti-hemoglobin ant
• Note: For research use only
• Application notes: Anti-Hemoglobin beta S (MOUSE) antibody has been tested by SDS-Page and western blot. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa in appropriate lysates.
• Expiration Date: 12 months from date of receipt.

Binding mode and kinetic analysis of Mouse Anti-HbS Antibody (p/n orb420236). Using Octet R4, a series of nine (9) concentrations of Mouse Anti-HbS Antibody (3.0 nM-2.2 μM) were immobilized on an AR2G biosensor and exposed to 20 µg/ml HbS protein. Each colored curve represents the acquired signal of its antibody concentration. The interaction features reproducible duplicate injections and fits to a 1:2 bivalent kinetic model. The association constant (ka = 32x103M-1s-1) and dissociation constant (kd = 8.74x10-4s-1) values are depicted in the graph. The assay was performed as per the manufacture's protocol.

Fitting of Kinetic data to bivalent binding site model. Steady state signals (blue filled symbols) are fitted to a bivalent binding site model (blue line). A 4-parameter non-linear regression curve fit was used for the integration of the data. The Mouse Anti-HbS Antibody (p/n orb420236) presents a KD = 27 nM (affinity constant) to HbS protein.

Lateral Flow Results of Anti-HbA (Hemoglobin A) and Anti-HbS (Hemoglobin beta S) Antibodies. Triplicate test strips are spotted with a control, Anti-HbA antibody (p/n orb420234) 0.5 µL at 250 ug/ml, and Anti-HbS antibody 0.5 µL at 1 mg/mL. Recombinant HbA (left group) or recombinant HbS (right group) are observed to react with the corresponding antibodies specifically, leading to blue dots.

Western blot results of Mouse Anti-HbS Antibody. Lane 1: Opal Prestained molecular weight ladder. Lane 2: HbA. Lane 3: HbA2. Lane 4: HbC. Lane 5: HbF. Lane 6: HbS. Lane 7: BSA. Loaded 10 ug. Blocking: BlockOut Universal buffer - orb348644 for 30 min at RT. Primary Antibody: Anti-Hemoglobin beta S at 1:1000 overnight at 4°C. Secondary Antibody: Rabbit Anti-Mouse HRP - orb347506 at 1:40000 for 30 min at RT.