Helicobacter pylori Catalase Antibody

• Catalog Number: orb1822654
• Category: Antibodies
• Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: HPYL/8548R
• Tested applications: IHC-P
• Reactivity: H. pylori
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant Helicobacter pylori Catalase protein fragment (within amino acids 323-445) was used as the immunogen for the Helicobacter pylori antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Helicobacter pylori antibody is available for research use only.
• Storage: Aliquot the Helicobacter pylori antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human H. pylori-infected stomach tissue with Helicobacter pylori antibody (clone HPYL/8548R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Helicobacter pylori antibody (clone HPYL/8548R) as confirmation of integrity and purity.