Heavy chain cardiac Myosin Rabbit Polyclonal Antibody (BF488)

• Catalog Number: orb2506049
• Category: Antibodies
• Description: Heavy chain cardiac Myosin Rabbit Polyclonal Antibody (BF488)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MYH6 / MYH7 (1801-1939/1939aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IF=1:100-500, ICC/IF=1:100-500
• Conjugation: BF488
• Target: MYH7
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: MYH6 + MYH7; MYH6 / MYH7; Alpha MHC; ASD3; CMD1S;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: U937. Primary Antibody (green line): Rabbit Anti-MYH7 antibody, dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.