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Catalog Number | orb18862 |
---|---|
Category | Antibodies |
Description | HDAC4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human HDAC4 recombinant protein (Position: Q4-Q213). Human HDAC4 shares 98.6% and 98.1% amino acid (aa) sequence identity with mouse and rat HDAC4, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 119040 MW |
UniProt ID | P56524 |
RRID | AB_10755594 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Histone deacetylase 4;HD4;3.5.1.98;HDAC4;KIAA0288; Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of THP-1 cells using anti-HDAC4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of SiHa cells using anti-HDAC4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of HDAC4 using anti-HDAC4 antibody.Lane 1:human HEK293 cell; 2:human HELA cell; 3:human THP-1 cell; 4:human HEPG2 cell; 5:human U87 cell; 6:rat testis tissue; 7:mouse testis tissue; 8:mouse NIH/3T3 cell.
IF analysis of HDAC4 using anti-HDAC4 antibody. HDAC4 was detected in immunocytochemical section of MCF-7 cells.
IHC analysis of HDAC4 using anti-HDAC4 antibody. HDAC4 was detected in paraffin-embedded section of human bladder cancer tissue.
IHC analysis of HDAC4 using anti-HDAC4 antibody. HDAC4 was detected in paraffin-embedded section of human pancreatic cancer tissue.
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