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Catalog Number | orb1274572 |
---|---|
Category | Antibodies |
Description | HDAC1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 62kDa |
Target | HDAC1 |
UniProt ID | Q13547 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | HDAC1 Antibody: HD1, RPD3, GON-10, RPD3L1, Histone Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using HDAC1 antibody at 1:1000 dilution.
Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HDAC1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using HDAC1 antibody. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug HDAC1 antibody. Western blot was performed from the immunoprecipitate using HDAC1 antibody at a dilition of 1:1000.
ELISA, IF, IHC, Multiplex Assay, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, Multiplex Assay, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Yeast, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, ELISA, IF, WB | |
Canine, Rat | |
Human, Mouse | |
Goat | |
Polyclonal | |
Unconjugated |
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