HCA66 Antibody

• Catalog Number: orb1526383
• Category: Antibodies
• Description: HCA66 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: Between 547 and C-terminus
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. A302-575A has not performed satisfactorily when used for WB of HCA66 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of HCA66.; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: HCA66
• UniProt ID: Q9NYH9
• NCBI: NP_060898.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: hepatocellular carcinoma-associated antigen 66; UT
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human HCA66 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-HCA66 (Cat. No. orb1526383) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human HCA66 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCA66 antibody orb1526383 used for IP at 3 µg/mg lysate.