HBsAg Goat Polyclonal Antibody

• Catalog Number: orb10771
• Category: Antibodies
• Description: HBsAg Goat Polyclonal Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Insect
• Reactivity: Human, Virus
• Isotype: IgG
• Immunogen: Recombinant HBsAg protein
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 44 kDa
• Target: C
• RRID: AB_10748308
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M PBS (pH7.4) with 0.02% Proclin300.
• Alternative names: L glycoprotein; L-HBsAg; LHB; Large S protein; Lar
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Measured by its binding ability in a indirect ELISA. Immobilized recombinant HBsAg protein at 2 µg/mL (100 µl/well) can bind Goat Anti-Human HBsAg Antibody, the EC50 is 29.14 ng/ml.

Paraformaldehyde-fixed, paraffin embedded (Hepatitis B virus infected human hepatocellular carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HBsAg) Polyclonal Antibody, Unconjugated (orb10771) at 1:200 overnight at 4°C, followed by A594 conjugated Rabbit Anti-Goat IgG H&L secondary antibody incubation and staining.

Sample: Lane 1: Recombinant HBsAg protein, Primary: Anti-HBsAg (orb10771) at 1/1000 dilution, Secondary: IRDye800CW Donkey anti-Goat IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 20 kDa.