HAUSP antibody

• Catalog Number: orb345449
• Category: Antibodies
• Description: HAUSP antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of human HAUSP protein.
• Concentration: 1.1 mg/mL
• Dilution range: ELISA: 1:15,000 - 1:60,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human HAUSP protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with HAUSP protein from human, rat, mouse, chimpanzee, bovine and dog based on 100% homology with the immunizing sequence. Expect partial reactivity with HAUSP from chicken (92%, 11/12) and zebrafish (83%, 10/12) sources based on partial sequence homology. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q93009
• NCBI: 150378533
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-HAUSP antibody, USP7, Deubiquitinating
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to HAUSP protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

ELISA results of purified Rabbit anti-HAUSP antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n orb347654) and TMB substrate (p/n orb348651).

Western blot using Biorbyt's affinity purified anti-HAUSP antibody shows detection of HAUSP in various cell lysates at 130 kDa. Lane 1: HeLa nuclear extract (p/n orb348671), Lane 2: HeLa (p/n orb348668), Lane 3: A431 (p/n orb348665), Lane 4: MCF7, Lane 5: 3T3 (orb348714). The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates. A 1:500 dilution of the primary antibody was used for detection followed by a 1:5000 dilution of HRP Gt-a-Rabbit IgG. Exposure time was 30 s.