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Catalog Number | orb1274453 |
---|---|
Category | Antibodies |
Description | HADHA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human HADHA (NP_000173.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 82kDa |
Target | HADHA |
UniProt ID | P40939 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Trifunctional enzyme subunit alpha, mitochondrial, Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using HADHA antibody (orb1274453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Western blot analysis of extracts from normal (control) and HADHA knockout (KO) 293T cells, using HADHA antibody (orb1274453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using HADHA antibody (orb1274453). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug HADHA antibody (orb1274453). Western blot was performed from the immunoprecipitate using HADHA antibody (orb1274453) at a dilution of 1:1000.
ELISA, IF, IHC-P, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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