HADHA Antibody, KO Validated

• Catalog Number: orb1274453
• Category: Antibodies
• Description: HADHA Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human HADHA (NP_000173.2).
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 82kDa
• Target: HADHA
• UniProt ID: P40939
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Trifunctional enzyme subunit alpha, mitochondrial,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HADHA antibody (orb1274453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Western blot analysis of extracts from normal (control) and HADHA knockout (KO) 293T cells, using HADHA antibody (orb1274453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of U2OS cells using HADHA antibody (orb1274453). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug HADHA antibody (orb1274453). Western blot was performed from the immunoprecipitate using HADHA antibody (orb1274453) at a dilution of 1:1000.