HADHA Antibody (C-term) [Knockout Validated]

• Catalog Number: orb1787819
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: 737-763 aa
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:500-1:2000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 83000 Da
• Target: This HADHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-763 amino acids from the C-terminal region of human HADHA.
• UniProt ID: P40939
• NCBI: NP_000173.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Trifunctional enzyme subunit alpha, mitochondrial,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

HADHA Antibody (C-term) western blot analysis in A431 cell line lysates (35 ug/lane). This demonstrates the HADHA antibody detected the HADHA protein (arrow).

HADHA Antibody (C-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HADHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-HADHA Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

A predominant 75 kDa band for the HEK293T wild type lysate was observed (3 ug/ml anti-HADHA) vs the predicted size of 83 kDa. The molecular weight discrepancy could be due to post-translationally modification of the target protein, a splice-variant form of the target protein, a partially degraded form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. A less intense band at 65 kDa was also A weak band only in the 20ug lane was observed for the knock out lysate, suggesting incomplete knockout of the target gene.