HA Tag Antibody

• Catalog Number: orb640090
• Category: Antibodies
• Description: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 16.43
• Tested applications: ELISA, FACS, IF, IHC-P, WB
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids YPYDVPDYA were used as the immunogen for this HA Tag antibody.
• Dilution range: ELISA (order BSA-free format for coating),Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This HA Tag antibody is available for research use only.
• Storage: Store the HA Tag antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of HA-tagged recombinant protein with HA Tag antibody (clone 16.43).

Immunofluorescent staining of PFA-fixed human MCF7 cells that have been transfected with an HA-tagged protein, with HA Tag antibody (clone 16.43, green) and Reddot nuclear stain (red).