HA Tag Antibody

• Catalog Number: orb27986
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DYKDDDDK Tag is used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest is an amino-terminal or carboxy-terminal fusion. It is a useful tool for the localization and characterization of DDDDK tagged proteins.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB0411
• Tested applications: ChIP, IF, WB
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:1000, IF/ICC: 1:10-50, ChIP: 1:100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Target: KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-Tag from influenza hemagglutinin protein anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Simkin, Dina et al. Andersen's syndrome mutants produce a knockdown of inwardly rectifying K+ channel in mouse skeletal muscle in vivo Cell Tissue Res, 371, 309-323 (2018)

PubMed: 29018970

Western blot analysis of Tag-HA. 2x Antibody (arrow) using rabbit polyclonal. 293T/17 cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with 41kDa HA tagged vector.

Western blot analysis of anti-HA tag pab in HA-tagged recombinant protein bacterial lysate.

ChIP was performed with 35S: HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions.

Western blot analysis of lysate from 12 tag recombinant protein cell line, using Tag-HA. 2x Antibody. Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.