HA Tag Antibody

• Catalog Number: orb167085
• Category: Antibodies
• Description: Mouse monoclonal antibody to HA Tag.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 12CA5
• Tested applications: WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Dilution range: WB: 1:1000
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Target: KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti Tag from influenza hemagglutinin protein anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-HA Tag Antibody at 1: 8000 dilution + 12tag recombinant protein. Lysates/proteins at 20ng per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.