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Catalog Number | orb1294277 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to HA tag |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide (YPYDVPDYA) |
Dilution range | Western Blot 1:3000-1:7000 Immunofluorescence 1:500-1:1000 |
Conjugation | Unconjugated |
Target | HA tag |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti HA-tag antibody at 1/5000 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294277 overnight at 4°C.
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