H3K36me2 Antibody

• Catalog Number: orb1258183
• Category: Antibodies
• Description: H3K36me2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Other, Rat
• Isotype: IgG
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K36 of human histone H3
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 18kDa
• Target: H3K36me2
• UniProt ID: Q16695
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: H3F3A, H3t, H3.4, H3/g, H3FT
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K36 antibody (orb1258183). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K36 antibody (orb1258183).

Immunohistochemistry of paraffin-embedded human breast using DiMethyl-Histone H3-K36 antibody (orb1258183) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K36 antibody (orb1258183) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K36 antibody (orb1258183) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K36 antibody (orb1258183). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K36 antibody (orb1258183) and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.